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b3 primers  (ATCC)


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    Structured Review

    ATCC b3 primers
    B3 Primers, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b3+primers/B-3/pmc12943657-49-22-58
    Average 97 stars, based on 648 article reviews
    b3 primers - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Bacteria:

    Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection
    Article Snippet: To assess the specificity of the constructed LAMP system to detect M. marinum , we performed the same experiment using a Loopamp DNA Amplification Kit (Eiken Chemical) with cell lysates from clinical isolates of M. marinum , other Mycobacterium spp., and pathogenic bacteria causing skin infections ( ). .. The reaction mixture was prepared by adding 12.5 μL of 2× Reaction Mix (40 mM Tris-HCl [pH 8.8], 20 mM KCl, 16 mM MgSO 4 , 20 mM (NH 4 ) 2 SO 4 , 0.2% Tween20, 1.6 M Betaine, and 2.8 mM/each dNTP), 40 pmol of FIP and BIP primers, 5 pmol of F3 and B3 primers, 1 μL of Bst DNA Polymerase, 120 μM HNB (hydroxy naphthol blue) (Dojindo Laboratories, Tokyo, Japan), 50 ng of template DNA (for M. marinum ATCC 927) or 2 μL of 10-fold-diluted cell lysates (for bacteria other than M. marinum ATCC 927), and sterile water to make a total volume of 25 μL. ..

    Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection.
    Article Snippet: To assess the specificity of the constructed LAMP system to detect M. marinum, we performed the same experiment using a Loopamp DNA Amplification Kit (Eiken Chemical) with cell lysates from clinical isolates of M. marinum, other Mycobacterium spp., and pathogenic bacteria causing skin infections (Table 1). .. The reaction mixture was prepared by adding 12.5 μL of 2× Reaction Mix (40 mM Tris-HCl [pH 8.8], 20 mM KCl, 16 mM MgSO4, 20 mM (NH4)2SO4, 0.2% Tween20, 1.6 M Betaine, and 2.8 mM/each dNTP), 40 pmol of FIP and BIP primers, 5 pmol of F3 and B3 primers, 1 μL of Bst DNA Polymerase, 120 μM HNB (hydroxy naphthol blue) (Dojindo Laboratories, Tokyo, Japan), 50 ng of template DNA (for M. marinum ATCC 927) or 2 μL of 10-fold-diluted cell lysates (for bacteria other than M. marinum ATCC 927), and sterile water to make a total volume of 25 μL. ..

    Sterility:

    Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection
    Article Snippet: To assess the specificity of the constructed LAMP system to detect M. marinum , we performed the same experiment using a Loopamp DNA Amplification Kit (Eiken Chemical) with cell lysates from clinical isolates of M. marinum , other Mycobacterium spp., and pathogenic bacteria causing skin infections ( ). .. The reaction mixture was prepared by adding 12.5 μL of 2× Reaction Mix (40 mM Tris-HCl [pH 8.8], 20 mM KCl, 16 mM MgSO 4 , 20 mM (NH 4 ) 2 SO 4 , 0.2% Tween20, 1.6 M Betaine, and 2.8 mM/each dNTP), 40 pmol of FIP and BIP primers, 5 pmol of F3 and B3 primers, 1 μL of Bst DNA Polymerase, 120 μM HNB (hydroxy naphthol blue) (Dojindo Laboratories, Tokyo, Japan), 50 ng of template DNA (for M. marinum ATCC 927) or 2 μL of 10-fold-diluted cell lysates (for bacteria other than M. marinum ATCC 927), and sterile water to make a total volume of 25 μL. ..

    Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection.
    Article Snippet: The reactions were performed using 2× LAMP Master Mix (Eiken Chemical, Tokyo, Japan). .. The reaction mixture was prepared by adding 12.5 μL of 2× Reaction Master Mix (Eiken Chemical), 40 pmol of FIP and BIP primers, 5 pmol of F3 and B3 primers, 50 ng of template DNA (from M. marinum ATCC 927 purified with the commercial kit), and sterile water to a final volume of 25 μL. ..

    Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection.
    Article Snippet: To assess the specificity of the constructed LAMP system to detect M. marinum, we performed the same experiment using a Loopamp DNA Amplification Kit (Eiken Chemical) with cell lysates from clinical isolates of M. marinum, other Mycobacterium spp., and pathogenic bacteria causing skin infections (Table 1). .. The reaction mixture was prepared by adding 12.5 μL of 2× Reaction Mix (40 mM Tris-HCl [pH 8.8], 20 mM KCl, 16 mM MgSO4, 20 mM (NH4)2SO4, 0.2% Tween20, 1.6 M Betaine, and 2.8 mM/each dNTP), 40 pmol of FIP and BIP primers, 5 pmol of F3 and B3 primers, 1 μL of Bst DNA Polymerase, 120 μM HNB (hydroxy naphthol blue) (Dojindo Laboratories, Tokyo, Japan), 50 ng of template DNA (for M. marinum ATCC 927) or 2 μL of 10-fold-diluted cell lysates (for bacteria other than M. marinum ATCC 927), and sterile water to make a total volume of 25 μL. ..

    Purification:

    Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection.
    Article Snippet: The reactions were performed using 2× LAMP Master Mix (Eiken Chemical, Tokyo, Japan). .. The reaction mixture was prepared by adding 12.5 μL of 2× Reaction Master Mix (Eiken Chemical), 40 pmol of FIP and BIP primers, 5 pmol of F3 and B3 primers, 50 ng of template DNA (from M. marinum ATCC 927 purified with the commercial kit), and sterile water to a final volume of 25 μL. ..

    Article Title: One-step loop-mediated isothermal amplification system for Mycobacterium marinum detection
    Article Snippet: The reactions were performed using 2× LAMP Master Mix (Eiken Chemical, Tokyo, Japan). .. The reaction mixture was prepared by adding 12.5 μL of 2× Reaction Master Mix (Eiken Chemical), 40 pmol of FIP and BIP primers, 5 pmol of F3 and B3 primers, 50 ng of template DNA (from M. marinum ATCC 927 purified with the commercial kit), and sterile water to a final volume of 25 μL. ..

    Polymerase Chain Reaction:

    Article Title: Effect of internal primer–template mismatches on loop-mediated isothermal amplification
    Article Snippet: .. The PCR was performed in a 20 mL reaction mixture with TaqMan® Universal Master Mix II (Life Technologies Corporation), according to the manufacturer’s instructions: 10 mL TaqMan® Universal Master Mix II, 0.5 mmol/L of F3 and B3 primers and 100 pg DNA template of S. agalactiae ATCC 27956. .. The reaction mixture was held at 50 C for 2 min and 95 C for 10 min, followed by 40 cycles of denaturation for 15 s at 95 C and 1 min anneal/extend at 60 C. The amplified products were sequenced by Sangon Biotech (Shanghai) Co. Ltd., and were then compared with the sequence of S. agalactiae strain GBS6 in GenBank.

    Article Title: Evaluation of LAMP Assay Using Phenotypic Tests and Conventional PCR for Detection of nuc and mecA genes Among Clinical Isolates of Staphylococcus spp
    Article Snippet: .. The specificity of the LAMP F3 and B3 primers were confirmed by PCR on the ATCC strains (positive and negative controls). .. [ ] shows the agarose gel image of the PCR result of the nuc and mecA genes, found only in the ATCC standard for MRSA and the nuc gene alone in the ATCC MSSA. fig ft0 fig mode=article f1 [Table/Fig-2]: caption a4 nuc and mecA conventional PCR agarose gel image by using LAMP outer (F3 B3) primers The specificity of the LAMP assays for the 2 genes were 100% on the ATCC strains.



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